Yersinia pestis DNA Detection Reagent Kit using Real-Time Polymerase Chain Reaction with Hybridization-Fluorescence Result Analysis (Yersinia pestis Gene Indication - RGF) as per TU 9398-037-01898109-2011

Yersinia pestis DNA Detection Reagent Kit using Real-Time Polymerase Chain Reaction with Hybridization-Fluorescence Result Analysis (Yersinia pestis Gene Indication - RGF) as per TU 9398-037-01898109-2011

The kit for detecting Yersinia pestis DNA via the polymerase chain reaction method with real-time hybridization-fluorescence result analysis consists of:

1. PCR Mix 1 - a clear colorless liquid containing: a mixture of two primers ("Syntol", Russia), each at a concentration of 8 pmol; a probe ("Syntol", Russia) at a concentration of 4 pmol; a mixture of deoxynucleoside triphosphates (dNTP) - dATP, dGTP, dCTP, dTTP (SibEnzyme, Russia), each at a concentration of 1 mmol; sodium azide ("Sigma", USA, or equivalent) - 0.031%.
2. PCR Mix 2 - a clear colorless liquid containing Tris-HCl - 167.5 mmol, pH 8.8 at 25°C (SibEnzyme, Russia); ammonium sulfate (SibEnzyme, Russia) - 41.5 mmol; magnesium chloride (SibEnzyme, Russia) - 5.0 mmol; Tween-20 (SibEnzyme, Russia) - 0.025%.
3. Taq Polymerase - a clear colorless liquid (SibEnzyme, Russia) - 5 units/µl.
4. TE Buffer - a clear colorless liquid containing Tris-HCl (Amresco, USA) - 10 mmol; Na2-EDTA (Amresco, USA) - 1 mmol.
5. Positive Control DNA Sample of Y. pestis (DNA PCO) - a clear colorless liquid representing an aqueous solution of Y. pestis DNA at a concentration of 1 ng.

To purchase the Yersinia pestis DNA Detection Reagent Kit employing the real-time PCR method, with hybridization-fluorescence result analysis according to TU 9398-037-01898109-2011, kindly place an order through our website by filling out a free-form request or using the provided order form. For inquiries, contact us via email at Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript..