Reagent Kit for Spoligotyping Human and Bovine Tuberculosis Mycobacteria by Hybridization on a Biological Microchip (Spoligo-Biochip) according to TU 9398-011-02699501-2009 (see Appendix on Page 1)

Reagent Kit for Spoligotyping Human and Bovine Tuberculosis Mycobacteria by Hybridization on a Biological Microchip (Spoligo-Biochip) according to TU 9398-011-02699501-2009 (see Appendix on Page 1)

Reagent Kit for Spoligotyping Human and Bovine Tuberculosis Mycobacteria by Hybridization on a Biological Microchip (Spoligo-Biochip) according to TU 9398-011-02699501-2009 (see Appendix on Page 1):

a) Set No. 1 for DNA extraction of Tuberculosis Complex Mycobacteria from biological material, including:

• Decont-A (Reagent A for sample decontamination) - 10 tubes (0.25 g each);
• Decont-B (Reagent B for sample decontamination) - 10 vials (50 ml each);
• WB-1 (Washing Buffer No. 1) - 5 vials (25 ml each);
• WB-2 (Washing Buffer No. 2) - 1 vial (20 ml);
• LB (Lysis Buffer) - 1 vial (10 ml);

b) Set No. 2 for PCR, including:

• PCR-Buffer (10x PCR Buffer with magnesium chloride) - 1 tube (1.5 ml);
• dNTPs (Aqueous solution of deoxynucleoside triphosphates for PCR) - 1 tube (1.4 ml);
• PR-1 (Aqueous solution of primers for PCR) - 1 tube (0.1 ml);
• PR-2 (Aqueous solution of fluorescently labeled primers for specific labeling of PCR product) - 1 tube (0.5 ml);
• Taq (Taq Polymerase) - 1 tube (0.2 ml);
• U (Aqueous solution of fluorescently labeled uracil nucleoside triphosphates) - 1 tube (0.05 ml);
• UDG (Uracil-DNA Glycosylase) - 1 tube (0.2 ml);
• BSA (Aqueous solution of Bovine Serum Albumin) - 1 tube (0.6 ml);
• K+ (Positive Control DNA sample of M. tuberculosis) - 1 tube (0.1 ml);
• K- (Negative Control sample) - 1 tube (1.5 ml);
• MM (Mineral Oil) - 1 vial (10 ml);
• MQ (Deionized Water for PCR) - 1 vial (10 ml);

c) Set No. 3 for hybridization on the biochip, including:

• HB (Hybridization Buffer) - 2 tubes (2.0 ml each);

d) Set No. 4, including biochips - 100 pieces.

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