Reagent Kit for Semi-quantitative Determination of Regulatory Autoantibodies in the Serum of Women of Childbearing Age "ELI-P-Complex" according to TU 9398-005-58201855-2008 in the Following Configuration (see attachment on 1 sheet):

Reagent Kit for Semi-quantitative Determination of Regulatory Autoantibodies in the Serum of Women of Childbearing Age "ELI-P-Complex" according to TU 9398-005-58201855-2008 in the Following Configuration (see attachment on 1 sheet):

1. Control Serum based on Human Blood Serum with Known Antibody (AB) Levels to Antigens 1...8 (Laboratory Production Regulation No. 11, Immunculus Medical Research Center), mixed with Glycerin (GOST 6259-75) in a volume ratio of 1:1 - 1 tube (0.25 ml); the control serum is certified compared to Human IgG (Liquid) (cat. No. I 2511, manufactured by Sigma, USA);
2. Buffer for Sample Dilution [2.4 g Sodium Chloride (GOST 4233-77), 0.022 g Monopotassium Phosphate (GOST 4198-75), 1.125 g Disodium Phosphate 12-Water (GOST 4172-76), and 0.06 g Potassium Chloride (GOST 4234-77)] - 1 packet;
3. Buffer for Washing Tablets [24.3 g Sodium Chloride (GOST 4233-77), 0.022 g Monopotassium Phosphate (GOST 4198-75), 1.125 g Disodium Phosphate 12-Water (GOST 4172-76), and 0.06 g Potassium Chloride (GOST 4234-77)] - 1 packet;
4. Reaction Developing Buffer (without Hydrogen Peroxide) [0.73 g Citric Acid Monohydrate (TU 6-09-584-75) and 1.19 g Anhydrous Disodium Phosphate (manufactured by Sigma, USA, cat. No. S 0876)] - 1 packet;
5. 3% Hydrogen Peroxide Solution [USSR Pharmacopeia, 3rd ed., 1968, p. 621] - 1 Eppendorf tube (0.3 ml);
6. Stop Solution [1M Sulfuric Acid (GOST 667-73)] - 3 bottles (10 ml each);
7. 10% Aqueous Solution of Tween-20 (manufactured by Serva, Germany, cat. No. 37470) - 1 bottle (12 ml);
8. Conjugate of Polyclonal Rabbit Antibodies to Human IgG with Horseradish Peroxidase (Production Regulation No. 32, IMTEK LLC, Moscow), Kdiss = 10^-9 mol/L - 2 tubes (0.060 ml each);
9. 0.2% Tetramethylbenzidine Solution in 75% Aqueous Solution of Dimethyl Sulfoxide (3,3', 5,5'-tetramethylbenzidine (TMB) (cat. No. T 5513, manufactured by Sigma, USA; packaging DIA-M) - 2 tubes (1 ml each);
10. Tablet with Antigens Sorbed on the Surface of Wells 96-well Polystyrene with Lid (F96 No 439454 Certif.maxisorp; manufactured by Nunk, Denmark) - 10 pieces.

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