Reagent Kit for Rapid Identification of Francisella tularensis Strains by Multilocus Polymerase Chain Reaction with Electrophoretic Result Analysis (Gene Francisella tularensis - subspecies - REF) according to TU 9398-042-01898109-2012

A reagent kit for rapid identification of Francisella tularensis strains by multilocus polymerase chain reaction with electrophoretic result analysis (Gene Francisella tularensis - subspecies - REF) includes:

PCR Mix-1 - clear colorless liquid containing:
- Four primers mix ("Sintol", Russia), each at a concentration of 10 pmol
- Deoxynucleoside triphosphate mix (dATP, dGTP, dCTP, dTTP) ("SibEnzyme", Russia), each at a concentration of 1 mmol
- Sodium azide ("Sigma", USA) - 0.031%
PCR Mix-2 - clear purple liquid containing:
- Tris-HCl - 167.5 mmol, pH 8.8 at 25 °C ("SibEnzyme", Russia)
- Ammonium sulfate ("SibEnzyme", Russia) - 41.5 mmol
- Magnesium chloride ("SibEnzyme", Russia) - 5.0 mmol
- Tween-20 ("SibEnzyme", Russia) - 0.025%
- Glycerol ("Amresco", USA) - 1%
- Bromophenol blue sodium salt ("Amresco", USA) - 0.01%
Hot Start Taq DNA Polymerase - clear colorless liquid ("SibEnzyme", Russia) - 5 units/µl
TE Buffer - clear colorless liquid containing:
- Tris-HCl ("Amresco", USA) - 10 mmol
- Na2-EDTA ("Amresco", USA) - 1 mmol
Mineral Oil - clear viscous liquid, light white paraffin mineral oil ("AppliChem", USA)
Positive Control DNA Sample F. tularensis "1670c" - clear colorless liquid, aqueous solution of F. tularensis tularensis DNA at a concentration of 1 ng/ml (Federal State Budgetary Institution "Microbe" of Rospotrebnadzor)
Positive Control DNA Sample F. tularensis "ISFtu2" - clear colorless liquid, aqueous solution of F. tularensis holarctica DNA at a concentration of 1 ng/ml (Federal State Budgetary Institution "Microbe" of Rospotrebnadzor)

To purchase the Reagent Kit for Rapid Identification of Francisella tularensis Strains by Multilocus Polymerase Chain Reaction with Electrophoretic Result Analysis (Gene Francisella tularensis - subspecies - REF) according to TU 9398-042-01898109-2012, please fill out the application form on our website and send it to Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript., or use the order form available on the website.

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