Reagent Kit for Identification of Brucella spp. Strains by Polymerase Chain Reaction Method with Hybridization-Fluorescence Detection in Real-Time Mode (Brucella Gene - Identification - HFD) according to TU 9398-045-01898109-2013

Reagent Kit for Identification of Brucella spp. Strains by Polymerase Chain Reaction Method with Hybridization-Fluorescence Detection in Real-Time Mode (Brucella Gene - Identification - HFD) according to TU 9398-045-01898109-2013

The reagent kit for the identification of Brucella spp. strains by polymerase chain reaction (PCR) method with hybridization-fluorescence detection in real-time mode (Brucella Gene - Identification - HFD) according to TU 9398-045-01898109-2013 includes:

1. PCR-Mixture-1 - a clear, colorless liquid containing:
   • A mixture of four primers (Syntol, Russia), each at a concentration of 8 pmol;
   • Two probes (Syntol, Russia) at a concentration of 4 pmol each;
   • A mixture of deoxynucleoside triphosphates (dNTPs) - dATP, dGTP, dCTP, dTTP (SibEnzyme, Russia), each at a concentration of 1 mmol;
   • Sodium azide (Sigma, USA, or equivalent) - 0.031%.
2. PCR-Mixture-2 - a clear, colorless liquid containing:
   • A mixture of two primers (Syntol, Russia), each at a concentration of 8 pmol;
   • One probe (Syntol, Russia) at a concentration of 4 pmol;
   • A mixture of deoxynucleoside triphosphates (dNTPs) - dATP, dGTP, dCTP, dTTP (SibEnzyme, Russia), each at a concentration of 1 mmol;
   • Sodium azide (Sigma, USA, or equivalent) - 0.031%.
3. PCR-Mixture-3 - a clear, colorless liquid containing:
   • Tris-HCl - 167.5 mmol, pH 8.8 at 25°C (SibEnzyme, Russia);
   • Ammonium sulfate (SibEnzyme, Russia) - 41.5 mmol;
   • Magnesium chloride (SibEnzyme, Russia) - 5.0 mmol;
   • Tween-20 (SibEnzyme, Russia) - 0.025%;
   • Sodium azide (Sigma, USA, or equivalent) - 0.031%.
4. Taq DNA polymerase - a clear, colorless liquid (SibEnzyme, Russia) - 5 U/µl;
5. TE buffer - a clear, colorless liquid containing:
   • Tris-HCl (Amresco, USA) - 10 mmol;
   • Na2-EDTA (Amresco, USA) - 1 mmol.
6. H₂O - a clear, colorless liquid, nuclease-free (Fermentas, USA);
7. Positive control sample of Brucella DNA (PCS Brucella DNA) - a clear, colorless liquid representing an aqueous solution of B.suis 1330 DNA at a concentration of 1 ng/ml.

To purchase the reagent kit for the identification of Brucella spp. strains by polymerase chain reaction method with hybridization-fluorescence detection in real-time mode (Brucella Gene - Identification - HFD) according to TU 9398-045-01898109-2013, you can fill out an application in free form and send it to Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript. or use the order form on our website.