Reagent Kit for Hepatitis C Virus (HCV) RNA Detection in Serum or Plasma by Polymerase Chain Reaction (BIOTITR-C) according to TU 9398-064-17253567-2011, consisting of (see Attachment on 1 page):

Reagent Kit for Hepatitis C Virus (HCV) RNA Detection in Serum or Plasma by Polymerase Chain Reaction (BIOTITR-C) according to TU 9398-064-17253567-2011, consisting of (see Attachment on 1 page):

Reagent Kit for Hepatitis C Virus (HCV) RNA Detection in Serum or Plasma by Polymerase Chain Reaction (BIOTITR-C) according to TU 9398-064-17253567-2011, consisting of (see Attachment on 1 page):

I. Sample Preparation Kit (RNA extraction) in the following formats:

1. "Qualitative" format, including:
   • RNA-EXPRESS reagent containing an internal control sample (ICS);
   • Negative control sample based on bovine serum, lacking HCV RNA sequences;
   • Positive control sample based on bovine serum, containing HCV RNA sequences;
2. "Quantitative" format, including:
   • RNA-EXPRESS reagent containing an internal control sample (ICS);
   • Negative control sample based on bovine serum, lacking HCV RNA sequences;
   • Set of standard control samples (calibration samples) based on bovine serum, containing calibrated HCV RNA with known concentrations: 10^3, 10^4, 10^5, 10^6 copies of RNA/mL;

II. Reagent Kit for PCR amplification, including:

• Reaction mixture containing fluorescent probes;
• Taq polymerase;
• Diluent;
• Reverse transcriptase;
• Ribonuclease inhibitor;
• Dithiothreitol;
• PCR positive control.

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