Reagent Kit for Determining Lethal Factor of Siberian Ulcer Pathogen by Immuno-PCR (IPCR-LF Test System) according to TU 9398-187-78095326-2013
Antibodies to the lethal factor of Siberian ulcer pathogen (1 mg/mL) – 1 vial (100 µL).
Biotinylated antibodies to the lethal factor of Siberian ulcer pathogen (1 mg/mL), conjugated with biotin – 1 vial (100 µL).
Blocking solution – 1 bottle (30 mL).
Buffer solution I – 1 bottle (20 mL).
Buffer solution II – 1 bottle (30 mL).
Conjugate of biotinylated DNA with streptavidin – 1 vial (100 µL).
Control DNA matrix for real-time PCR amplification – 1 vial (10 µL).
Control sample of recombinant protein of Siberian ulcer pathogen's lethal factor (0.01 mg/mL) – 1 vial (100 µL).
Tween-20 – 1 vial (300 µL).
Primer 1 for real-time PCR amplification 5 pmol – 1 vial (300 µL).
Primer 2 for real-time PCR amplification 5 pmol – 1 vial (150 µL).
Restriction endonuclease SacI, 10 units/µL – 1 vial (30 µL).
SacI endonuclease buffer – 1 vial (500 µL).
TaqMan probe 5 pmol – 1 vial (125 µL).
PCR amplification buffer – 1 vial (250 µL).
Deoxynucleoside triphosphates – 1 vial (250 µL).
Taq DNA polymerase, 5 U/µL – 1 vial (30 µL).
MgCl2 solution, 25 mM – 1 vial (250 µL).
Deionized water – 1 vial (1.7 mL).
Immunological plate – 1 piece.
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