Reagent Kit for Detection of RNA Yersinia pestis/Francisella tularensis by Real-Time PCR with Hybridization-Fluorescent Detection (Gene Yersinia pestis/Francisella tularensis Detection - RT-PCR)

Reagent Kit for Detection of RNA Yersinia pestis/Francisella tularensis by Real-Time PCR with Hybridization-Fluorescent Detection (Gene Yersinia pestis/Francisella tularensis Detection - RT-PCR)

1. PCR Mix 1 - clear colorless liquid, containing:
   • Mixture of four primers ("Sintol", Russia), each at 8 pmol concentration.
   • Two probes ("Sintol", Russia) at 4 pmol concentration.
   • Mixture of deoxynucleoside triphosphates (dNTPs) - dATP, dGTP, dCTP, dTTP, ("SibEnzyme", Russia), each at 1 mmol concentration.
   • Sodium azide ("Sigma", USA, or equivalent) - 0.031%.
2. PCR Mix 2 - clear colorless liquid, containing:
   • Tris-HCl - 167.5 mmol, pH 8.8 at 25°C ("SibEnzyme", Russia).
   • Ammonium sulfate ("SibEnzyme", Russia) - 41.5 mmol.
   • Magnesium chloride ("SibEnzyme", Russia) - 5.0 mmol.
   • Tween-20 ("SibEnzyme", Russia) - 0.025%.
3. Taq Polymerase - clear colorless liquid ("SibEnzyme", Russia) - 5 units/µL.
4. TE Buffer - clear colorless liquid, containing:
   • Tris-HCl ("Amresco", USA) - 10 mmol.
   • Na2-EDTA ("Amresco", USA) - 1 mmol.
5. Nuclease-free Water - clear colorless liquid ("Fermentas", USA).
6. DNase - clear colorless liquid ("Promega", USA) - 1 unit/µL.
7. DNase Reaction Buffer - clear colorless liquid, containing:
   • Tris-HCl (pH 8.0) ("Promega", USA) - 400 mmol.
   • Magnesium sulfate ("Promega", USA) - 100 mmol.
   • Calcium chloride ("Promega", USA) - 10 mmol.
8. Stop Buffer for DNase - clear colorless liquid, containing:
   • EDTA (pH 8.0) ("Promega", USA) - 20 mmol.
9. Positive Control DNA Sample Y. pestis (PCO DNA Y. pestis) - clear colorless liquid, representing an aqueous solution of Y. pestis DNA at a concentration of 1 ng.
10. Positive Control DNA Sample F. tularensis (PCO DNA F. tularensis) - clear colorless liquid, representing an aqueous solution of F. tularensis DNA at a concentration of 1 ng.

Buy Reagent Kit for Detection of RNA Yersinia pestis/Francisella tularensis by Real-Time PCR with Hybridization-Fluorescent Detection (Gene Yersinia pestis/Francisella tularensis Detection - RT-PCR) according to TU 9398-047-01898109-2013. To order on our website, you can fill out a free-form application to Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript. or use the order form on the website.