Medical Technologies

Reagent Kit for Detection of DNA of Yersinia pestis by Real-Time PCR (GenPest - Indication - RGF) per TU 9398-050-01898109-2014

Reagent Kit for Detection of DNA of Yersinia pestis by Real-Time PCR (GenPest - Indication - RGF) per TU 9398-050-01898109-2014

  • PCR Mix-1 - dry, pink-colored precipitate, containing: a mixture of two primers, produced by ZAO "Sintol", Russia, each at a concentration of 8 pmol/μL; probe, produced by ZAO "Sintol", Russia, at a concentration of 4 pmol/μL; a mixture of deoxynucleoside triphosphates (dNTPs) - dATP, dGTP, dCTP, dTTP, produced by ZAO "Sintol", Russia, each at a concentration of 1 mmol/μL; sodium azide ("Amresco", USA) - 3 mmol/μL;
  • PCR Mix-RGF - clear colorless liquid, containing Tris-HCl - 125 mmol/L, pH 8.8 at 25°C, produced by ZAO "Sintol", Russia; potassium chloride, produced by ZAO "Sintol", Russia - 125 mmol/L; magnesium chloride, produced by ZAO "Sintol", Russia - 5.0 mmol/L; Tween-20, produced by ZAO "Sintol", Russia - 0.05 mmol/L; glycerol, produced by "Amresco", USA - 17 mmol/L; sodium azide, produced by "Amresco", USA - 3.75 mmol/L;
  • Taq DNA Polymerase - Syn Taq DNA Polymerase with enzyme inhibitory antibodies, clear colorless liquid, produced by ZAO "Sintol", Russia - 5 units/μL;
  • Solvent - clear colorless liquid, consisting of H2O, free from nucleases, produced by "Fermentas", USA;
  • TE Buffer - clear colorless liquid, containing Tris-HCl, produced by "Amresco", USA - 10 mmol/L; Na2-EDTA, produced by "Amresco", USA - 1 mmol/L;
  • Positive Control DNA Sample of Y. pestis (PCO DNA Y. pestis) - dry, as a trace of dried drop at the bottom of the tube, consisting of DNA of Y. pestis, isolated from a suspension of plague microbe at a concentration of 1×106 CFU/mL.

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