Reagent Kit for Detection of DNA of Francisella tularensis by Real-Time PCR (GenTul - Indication - RGF) per TU 9398-049-01898109-2014

PCR Mix-1 - dry, lilac-colored precipitate, containing: a mixture of two primers (ZAO "Sintol", Russia), each at a concentration of 8 pmol/μL; probe (ZAO "Sintol", Russia) at a concentration of 4 pmol/μL; a mixture of deoxynucleoside triphosphates (dNTPs) - dATP, dGTP, dCTP, dTTP (ZAO "Sintol", Russia), each at a concentration of 1 mmol/μL; sodium azide ("Amresco", USA) - 3 mmol/μL;
PCR Mix-RGF - clear violet liquid, containing Tris-HCl - 125 mmol/L, pH 8.8 at 25°C (ZAO "Sintol", Russia); potassium chloride (ZAO "Sintol", Russia) - 125 mmol/L; magnesium chloride (ZAO "Sintol", Russia) - 5.0 mmol/L; Tween-20 (ZAO "Sintol", Russia) - 0.05 mmol/L; glycerol ("Amresco", USA) - 17 mmol/L; sodium azide ("Amresco", USA) - 3.75 mmol/L;
Taq DNA Polymerase - Syn Taq DNA Polymerase with enzyme inhibitory antibodies, clear colorless liquid (ZAO "Sintol", Russia) - 5 units/μL;
Solvent - clear colorless liquid, consisting of H2O, free from nucleases ("Fermentas", USA);
TE Buffer - clear colorless liquid, containing Tris-HCl ("Amresco", USA) - 10 mmol/L; Na2-EDTA ("Amresco", USA) - 1 mmol/L;
Positive Control DNA Sample of F. tularensis (PCO DNA F. tularensis) - dry, as a trace of dried drop at the bottom of the tube, consisting of DNA of F. tularensis, isolated from a suspension of tularemia microbe at a concentration of 1×106 CFU/mL.

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