Reagent Kit for Detection of DNA of Francisella tularensis by Electrophoretic Real-Time PCR (GenTul - Indication - REF) per TU 9398-048-01898109-2014

PCR Mix-1 - dry, white precipitate, containing: a mixture of two primers ("Sintol", Russia), each at a concentration of 10 pmol/μL; a mixture of deoxynucleoside triphosphates (dNTPs) - dATP, dGTP, dCTP, dTTP ("Sintol", Russia), each at a concentration of 1 mmol/μL; sodium azide ("Amresco", USA) - 3 mmol/μL;
PCR Mix-REF - clear purple liquid, containing Tris-HCl - 125 mmol/L, pH 8.8 at 25°C ("Sintol", Russia); potassium chloride ("Sintol", Russia) - 125 mmol/L; magnesium chloride ("Sintol", Russia) - 5.0 mmol/L; Tween-20 ("Sintol", Russia) - 0.05 mmol/L; glycerol ("Amresco", USA) - 20.4 mmol/L; bromophenol blue sodium salt ("Amresco", USA) - 0.09 mmol/L; sodium azide ("Amresco", USA) - 3.75 mmol/L;
Taq DNA Polymerase - Syn Taq DNA Polymerase with enzyme inhibitory antibodies, clear colorless liquid ("Sintol", Russia) - 5 units/μL;
Solvent - clear colorless liquid, consisting of H2O, free from nucleases ("Fermentas", USA);
TE Buffer - clear colorless liquid, containing Tris-HCl, ("Amresco", USA) - 10 mmol/L; Na2-EDTA ("Amresco", USA) - 1 mmol/L;
Mineral Oil - clear viscous liquid, consisting of light white mineral oil, paraffin ("AppliChem", USA);
Positive Control DNA Sample of F. tularensis (PCO DNA F. tularensis) - dry, as a trace of dried drop at the bottom of the tube, consisting of DNA of F. tularensis, isolated from a suspension of tularemia microbe at a concentration of 1×106 CFU/mL.

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