Reagent Kit for Detecting Yersinia pestis DNA by PCR Method with Hybridization-Fluorescent Real-Time Results Detection (Gen Yersinia pestis Indication - RG-F) according to TU 9398-037-01898109-2011

Reagent Kit for Detecting Yersinia pestis DNA by PCR Method with Hybridization-Fluorescent Real-Time Results Detection (Gen Yersinia pestis Indication - RG-F) according to TU 9398-037-01898109-2011

The reagent kit for detecting Yersinia pestis DNA by PCR method with hybridization-fluorescent real-time results detection (Gen Yersinia pestis Indication - RG-F) according to TU 9398-037-01898109-2011 includes:

1. PCR Mix 1 - a clear, colorless liquid containing:
   • mixture of two primers ("Syntol", Russia), each at a concentration of 8 pmol;
   • probe ("Syntol", Russia) at a concentration of 4 pmol;
   • mixture of deoxynucleoside triphosphates (dNTP) - dATP, dGTP, dCTP, dTTP, ("SibEnzyme", Russia), each at a concentration of 1 mmol;
   • sodium azide ("Sigma", USA, or equivalent) - 0.031%.
2. PCR Mix 2 - a clear, colorless liquid containing:
   • Tris-HCl - 167.5 mmol, pH 8.8 at 25°C ("SibEnzyme", Russia);
   • ammonium sulfate ("SibEnzyme", Russia) - 41.5 mmol;
   • magnesium chloride ("SibEnzyme", Russia) - 5.0 mmol;
   • Tween-20 ("SibEnzyme", Russia) - 0.025%.
3. Taq Polymerase - a clear, colorless liquid ("SibEnzyme", Russia) - 5 units/µL.
4. TE Buffer - a clear, colorless liquid containing:
   • Tris-HCl ("Amresco", USA) - 10 mmol;
   • Na2-EDTA ("Amresco", USA) - 1 mmol.
5. Positive Control DNA Sample Y. pestis (PCO DNA) - a clear, colorless liquid, an aqueous solution of Y. pestis DNA at a concentration of 1 ng.

To purchase the reagent kit for detecting Yersinia pestis DNA by PCR method with hybridization-fluorescent real-time results detection (Gen Yersinia pestis Indication - RG-F) according to TU 9398-037-01898109-2011, please fill out the inquiry form on our website or send an email to Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript..