Reagent Kit for Detecting Francisella tularensis DNA by Real-Time Polymerase Chain Reaction with Hybridization-Fluorescent Detection (Gene Francisella tularensis-RT PCR) per TU 9398-035-01898109-2011

The reagent kit for detecting Francisella tularensis DNA by real-time polymerase chain reaction with hybridization-fluorescent detection (Gene Francisella tularensis-RT PCR) per TU 9398-035-01898109-2011 consists of:

PCR Mix-1 - Clear colorless liquid, containing a mixture of two primers ("Sintol", Russia), each at a concentration of 8 pmol; probe ("Sintol", Russia) at a concentration of 4 pmol; a mixture of deoxynucleoside triphosphates (dNTP) - dATP, dGTP, dCTP, dTTP ("SibEnzyme", Russia) each at a concentration of 1 mmol; sodium azide ("Sigma", USA, or equivalent) - 0.031%.
PCR Mix-2 - Clear colorless liquid, containing Tris-HCl - 187.5 mmol, pH 8.8 at 25°C (Fermentas, USA, or equivalent); ammonium sulfate (Fermentas, USA, or equivalent) - 50 mmol; magnesium chloride (Fermentas, USA, or equivalent) - 5.0 mmol; Tween-20 (Fermentas, USA, or equivalent) - 0.025%.
Taq DNA Polymerase - Clear colorless liquid (Fermentas, USA) - 5 units/µl.
TE Buffer - Clear colorless liquid, containing Tris-HCl (Amresco, USA) - 10 mmol; Na2-EDTA (Amresco, USA) - 1 mmol.
Positive control DNA sample of F. tularensis (PCO DNA F. tularensis) - Clear colorless liquid, representing an aqueous solution of F. tularensis DNA at a concentration of 1 ng.

To purchase the reagent kit for detecting Francisella tularensis DNA by real-time polymerase chain reaction with hybridization-fluorescent detection (Gene Francisella tularensis-RT PCR) per TU 9398-035-01898109-2011, you can fill out a request form on our website or use the order form. For inquiries, contact us via email at Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript..

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