Reagent Kit for Accelerated Identification of Francisella tularensis Strains by Multilocus Polymerase Chain Reaction with Hybridization-Fluorescent Real-Time Detection (Gene Francisella tularensis - Subspecies - RGF) According to TU 9398-043-01898109-2012

Reagent Kit for Accelerated Identification of Francisella tularensis Strains by Multilocus Polymerase Chain Reaction with Hybridization-Fluorescent Real-Time Detection (Gene Francisella tularensis - Subspecies - RGF) According to TU 9398-043-01898109-2012

The reagent kit for accelerated identification of Francisella tularensis strains by multilocus polymerase chain reaction with hybridization-fluorescent real-time detection (Gene Francisella tularensis - Subspecies - RGF) includes:

1. PCR Mix-1 - clear colorless liquid containing:
   • Four primers mix ("Sintol", Russia), each at a concentration of 8 pmol
   • Two probes ("Sintol", Russia) at a concentration of 4 pmol each
   • Mixture of deoxynucleoside triphosphates (dNTPs) - dATP, dGTP, dCTP, dTTP ("SibEnzyme", Russia), each at a concentration of 1 mmol
   • Sodium azide ("Sigma", USA, or equivalent) - 0.031%
2. PCR Mix-2 - clear colorless liquid containing:
   • Tris-HCl - 167.5 mmol, pH 8.8 at 25 °C ("SibEnzyme", Russia)
   • Ammonium sulfate ("SibEnzyme", Russia) - 41.5 mmol
   • Magnesium chloride ("SibEnzyme", Russia) - 5.0 mmol
   • Tween-20 ("SibEnzyme", Russia) - 0.025%
3. Hot Start Taq DNA Polymerase - clear colorless liquid (5 units/μl, "SibEnzyme", Russia)
4. TE Buffer - clear colorless liquid containing:
   • Tris-HCl ("Amresco", USA) - 10 mmol
   • Na2-EDTA ("Amresco", USA) - 1 mmol
5. Positive Control DNA Sample F. tularensis "1670c" (PCO DNA F. tularensis "1670c") - clear colorless liquid, aqueous solution of F. tularensis tularensis DNA at a concentration of 1 ng/ml (Federal State Institution "Microbe" of Rospotrebnadzor)
6. Positive Control DNA Sample F. tularensis "ISFtu2" (PCO DNA F. tularensis "ISFtu2") - clear colorless liquid, aqueous solution of F. tularensis holarctica DNA at a concentration of 1 ng/ml (Federal State Institution "Microbe" of Rospotrebnadzor)

To purchase the reagent kit for accelerated identification of Francisella tularensis strains by multilocus polymerase chain reaction with hybridization-fluorescent real-time detection (Gene Francisella tularensis - Subspecies - RGF) according to TU 9398-043-01898109-2012, please fill out the order form on our website or contact us via email at Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript..