Monoclonal Plague Microbe Immunoenzyme Test System (IFAPestF1-M) as per TU 9388-044-01898109-2012

Monoclonal Plague Microbe Immunoenzyme Test System (IFAPestF1-M) as per TU 9388-044-01898109-2012

The "IFAPestF1-M" test system includes:

1. Phosphate-buffered saline (PBS) ("PanEco", Russia) - white tablets, 5 pieces of 1.01 g each.
2. Defatted dry milk (lactalbumin) (GOST 10970-87) - cream-colored powder, 0.45 g.
3. Conjugate MKAF1* (conjugate peroxidase immunoglobulin monoclonal for plague) - amorphous brown mass, representing mouse monoclonal plague immunoglobulins conjugated with horseradish peroxidase, dried (Federal State Unitary Enterprise of the Russian National Institute for Plague Control of Rospotrebnadzor).
4. Glycerin ("Panreac", Spain) - viscous clear liquid, 0.05 ml.
5. Tween-20 ("Helicon", Russia) - slightly yellowish viscous liquid, 0.015 ml.
6. Citric acid ("AppliChem", Germany) - white powder, 0.18 g.
7. Sodium citrate ("AppliChem", Germany) - white powder, 0.20 g.
8. ABTS (2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), diammonium salt ("Sigma", Germany) - pale greenish powder, 0.0067 g.
9. Hydroperite (urea peroxide) ("Tatkhimfarmpreparaty", Russia) - white tablet, 1 piece of 1.5 g each.
10. Positive control sample (PCO-PM) - yellowish amorphous mass, representing an inactivated dry suspension of Y. pestis at a concentration of 1 x 10 9 CFU/ml (Federal State Unitary Enterprise of the Russian National Institute for Plague Control of Rospotrebnadzor).
11. Polystyrene plates for enzyme immunoassay ("Medpolimer", Russia), sensitized with monoclonal plague immunoglobulins at a concentration of 50 μg/ml, followed by lactalbumin blocking - 2 pieces.

The "IFAPestF1-M" test system is designed for duplicate analysis of 92 unknown samples and 2 control samples, totaling 192 determinations, with the placement of positive (1 well) and negative (3 wells) controls on each plate.

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