HIV-1 Genotyping Kit "TRUGENE HIV-1 Genotyping Kit" (see Appendix on 2 sheets)

HIV-1 Genotyping Kit "TRUGENE HIV-1 Genotyping Kit" (see Appendix on 2 sheets)

HIV-1 Genotyping Kit "TRUGENE HIV-1 Genotyping Kit", composition:

1. Box 1:
   1. Negative control. Buffer solution containing wheat embryo tRNA and TRIS-HCl buffer 1x100 ml - N - CTRL.
   2. RT-PCR primers 1x225 ml - RT-PCRPRIM.
   3. dNTP solution 1x60 ml - dNTP.
   4. RNase inhibitor. Buffer solution containing RNase inhibitor and glycerin 1x40 ml - RNaseIN.
   5. DTT solution. Solution containing 1.2% dithiothreitol (DTT) in water 1x50 ml - DTT.
   6. RT-PCR buffer. TRIS buffer solution containing magnesium chloride, ammonium sulfate, and acetylated bovine serum albumin 1x370 ml - RT-PCRBUF.
   7. RT enzyme. Buffer solution containing recombinant reverse transcriptase enzyme 1x37 ml - RT ENZ.
   8. DNA polymerase. TRIS buffer solution containing DNA polymerase 1x95 ml - DNA POL.
   9. CLIP enzyme solution 1x120 ml - CLIP ENZ.
   10. CLIP buffer. TRIS buffer solution containing magnesium chloride 1x630 ml - CLIP BUF.
   11. CLIP protease A mixture 1x220 ml - CLIP PROT-A.
   12. CLIP protease C mixture 1x220 ml - CLIP PROT-C.
   13. CLIP protease G mixture 1x220 ml - CLIP PROT-G.
   14. CLIP protease T mixture 1x220 ml - CLIP PROT-T.
   15. CLIP-P2 A mixture. Buffer solution 1x220 ml - CLIP P2-A.
   16. CLIP-P2 C mixture. Buffer solution 1x220 ml - CLIP P2-C.
   17. CLIP-P2 G mixture. Buffer solution 1x220 ml - CLIP P2-G.
   18. CLIP-P2 T mixture. Buffer solution 1x220 ml - CLIP P2-T.
   19. CLIP-RT Beginning A mixture. Buffer solution 1x220 ml - CLIP RTB-A.
   20. CLIP-RT Beginning C mixture. Buffer solution 1x220 ml - CLIP RTB-C.
   21. CLIP-RT Beginning G mixture. Buffer solution 1x220 ml - CLIP RTB-G.
   22. CLIP-RT Beginning T mixture. Buffer solution 1x220 ml - CLIP RTB-T.
   23. CLIP-RT Middle A mixture. Buffer solution 1x220 ml - CLIP RTM-A.
   24. CLIP-RT Middle C mixture. Buffer solution 1x220 ml - CLIP RTM-C.
   25. CLIP-RT Middle G mixture. Buffer solution 1x220 ml - CLIP RTM-G.
   26. CLIP-RT Middle T mixture. Buffer solution 1x220 ml - CLIP RTM-T.
   27. Stop solution with dye 1x7.1 ml - STOP.
2. Box 2:
   1. Positive control. Buffer solution 5x25 ml - P - CTRL.

Manufacturer address: "Siemens Healthcare Diagnostics Inc.", USA, Siemens Healthcare Diagnostics Inc., 333 Coney Street, East Walpole, Massachusetts 02032, USA.

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