DNA Detection Test System for Brucella spp. by Polymerase Chain Reaction Method (GenBru) According to TU 8895-008-01898109-2007

DNA Detection Test System for Brucella spp. by Polymerase Chain Reaction Method (GenBru) According to TU 8895-008-01898109-2007

The test system for detecting Brucella spp. DNA by the polymerase chain reaction method (GenBru) according to TU 8895-008-01898109-2007 includes:

1. Bru31 primers - a transparent colorless liquid, a water solution of oligonucleotide primers, concentration 12 pmol/μl (Federal State Budgetary Institution "Microbe"); 1 vial of 0.05 ml;
2. Bru32 primers - a transparent colorless liquid, a water solution of oligonucleotide primers, concentration 12 pmol/μl (Federal State Budgetary Institution "Microbe"); 1 vial of 0.05 ml;
3. Bru3 primers - a transparent colorless liquid, a water solution of oligonucleotide primers, concentration 12 pmol/μl (Federal State Budgetary Institution "Microbe"); 1 vial of 0.05 ml;
4. Bru4 primers - a transparent colorless liquid, a water solution of oligonucleotide primers, concentration 12 pmol/μl (Federal State Budgetary Institution "Microbe"); 1 vial of 0.05 ml;
5. Taq polymerase - a transparent colorless liquid, a thermostable DNA polymerase in a buffer solution, recombinant ("InterLabService," Russia) - 5 units/μl; 1 vial of 0.02 ml;
6. dNTPs - a transparent colorless liquid, a water solution of deoxyribonucleoside triphosphates (dATP, dGTP, dCTP, dTTP), each at a concentration of 2 mmol (Medigen, Russia); 1 vial of 0.25 ml;
7. DNA (positive control) - a transparent colorless liquid, a water solution of DNA B. abortus 19 BA at a concentration of 1 ng/μl (Federal State Budgetary Institution "Microbe"); 1 vial of 0.25 ml;
8. 10xB buffer - a transparent colorless liquid, a 10-fold buffer solution containing Tris-(hydroxymethyl)aminomethane (Helicon, Russia) - 0.7 mol; ammonium sulfate (Helicon, Russia) - 0.2 mol; Tween 20 (Helicon, Russia) - 0.1%; bovine serum albumin (Helicon, Russia) - 0.02%; pH up to 8.8±0.05; 1 vial of 0.25 ml;
9. MgCl2 - a transparent colorless liquid, a solution of magnesium chloride (Helicon, Russia) - 1%; 1 vial of 0.1 ml;
10. H2O - a transparent colorless liquid, representing distilled water, 1 vial of 1.2 ml;
11. Oil - a colorless viscous liquid, representing light white mineral oil, paraffin (AppliChem, USA), 2 vials of 1.5 ml each.

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